8IA3
Crystal structure of human USF2 bHLHLZ domain in complex with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97843 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 78.847, 56.951, 232.118 |
Unit cell angles | 90.00, 90.92, 90.00 |
Refinement procedure
Resolution | 37.140 - 3.500 |
R-factor | 0.2452 |
Rwork | 0.242 |
R-free | 0.29740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.737 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.13) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.010 | 3.830 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.170 | 0.811 |
Rmeas | 0.200 | 0.954 |
Rpim | 0.103 | 0.493 |
Total number of observations | 47261 | 10960 |
Number of reflections | 13068 | 3062 |
<I/σ(I)> | 5.2 | 1.6 |
Completeness [%] | 98.4 | |
Redundancy | 3.6 | 3.6 |
CC(1/2) | 0.993 | 0.820 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | Proplex-C5 0.1M Tris, PH 8.0, 20% w/v PEG4000 |