8I7X
Crystal structure of human ClpP in complex with ZG36
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 113.240, 96.080, 127.000 |
Unit cell angles | 90.00, 92.72, 90.00 |
Refinement procedure
Resolution | 30.850 - 1.990 |
R-factor | 0.23253 |
Rwork | 0.231 |
R-free | 0.25974 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.110 |
Data reduction software | autoPROC |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.850 | 2.040 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.178 | 1.061 |
Number of reflections | 184458 | 13737 |
<I/σ(I)> | 13.7 | |
Completeness [%] | 99.1 | |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M sodium bromide, 20% (w/v) polyethylene glycol 3350 |