8I17
Structural basis for H2A-H2B recognitions by human Spt16
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97849 |
Spacegroup name | P 1 |
Unit cell lengths | 46.852, 49.084, 85.195 |
Unit cell angles | 85.95, 74.31, 61.80 |
Refinement procedure
Resolution | 40.191 - 1.980 |
R-factor | 0.1724 |
Rwork | 0.170 |
R-free | 0.21110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aoi |
RMSD bond length | 0.006 |
RMSD bond angle | 0.721 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (Phenix1.19.2-4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.010 |
High resolution limit [Å] | 1.980 | 5.370 | 1.980 |
Rmerge | 0.050 | 0.039 | 0.510 |
Rmeas | 0.060 | ||
Rpim | 0.033 | ||
Number of reflections | 42483 | 2100 | 1896 |
<I/σ(I)> | 24.3 | ||
Completeness [%] | 94.8 | 93.7 | 86.6 |
Redundancy | 2.9 | 3.1 | 2.5 |
CC(1/2) | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M MES monohydrate pH 6.0, 14% w/v Polyethylene glycol 4,000 |