8HYK
CD-NTase EfCdnE in complex with intermediate pppU[2'-5']p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 07A |
| Synchrotron site | NSRRC |
| Beamline | TPS 07A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-21 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97626 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.418, 56.940, 64.812 |
| Unit cell angles | 90.00, 97.02, 90.00 |
Refinement procedure
| Resolution | 28.486 - 2.021 |
| Rwork | 0.165 |
| R-free | 0.21020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.453 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.090 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.067 | 0.162 |
| Number of reflections | 19291 | 1849 |
| <I/σ(I)> | 24.5 | |
| Completeness [%] | 97.8 | 94.8 |
| Redundancy | 5.9 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | sodium tartrate, PEG 3350, MgCl2, UTP |
