8HYF

Crystal Structure of Banana Lectin In-complex with Fucose at 2.95 A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER D8 QUEST
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-11
Detector	Bruker PHOTON II
Wavelength(s)	1.5406
Spacegroup name	P 32 2 1
Unit cell lengths	81.219, 81.219, 147.315
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	16.960 - 2.950
R-factor	0.18748
Rwork	0.184
R-free	0.24216
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2bmy
RMSD bond length	0.009
RMSD bond angle	1.872
Data reduction software	PROTEUM PLUS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.960	2.950
High resolution limit [Å]	2.950	2.950
Rmerge	0.144	0.422
Rmeas	0.203	0.596
Number of reflections	12279	1932
<I/σ(I)>	9.2	2.4
Completeness [%]	99.7
Redundancy	1.9
CC(1/2)	0.953	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.25	293	1.275 M AMMONIUM SULFATE, 30 mM CADMIUM CHLORIDE, 100 mM TRIS-HCl, PH=8.25