8HUG

F1 in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	105.570, 105.570, 304.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.840 - 2.150
R-factor	0.1886
Rwork	0.187
R-free	0.22430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cnd
RMSD bond length	0.007
RMSD bond angle	1.419
Data reduction software	HKL-3000
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.840	30.840	2.210
High resolution limit [Å]	2.150	9.620	2.150
Rmerge	0.095	0.044	1.714
Rmeas	0.097	0.045	1.746
Rpim	0.020	0.010	0.335
Total number of observations	2352015	22646	187391
Number of reflections	93392	1205	6891
<I/σ(I)>	21.5	47.7	2.5
Completeness [%]	98.9	97	100
Redundancy	25.2	18.8	27.2
CC(1/2)	0.999	0.999	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.12 M Monosaccharides (20 mM D-Glucose; 20 mM D-Mannose; 20 mM D-Galactose; 20 mM L-Fucose; 20 mM D-Xylose; 20 mM N-Acetyl-D-Glucosamine), 0.1 M buffer system 1 pH 6.5 (sodium HEPES and MOPS), and 50 % Precipitant Mix 2 (40% v/v Ethylene glycol; 20 % w/v PEG 8000)