8HUG
F1 in complex with CRM1-Ran-RanBP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 105.570, 105.570, 304.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.840 - 2.150 |
| R-factor | 0.1886 |
| Rwork | 0.187 |
| R-free | 0.22430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7cnd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.419 |
| Data reduction software | HKL-3000 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.840 | 30.840 | 2.210 |
| High resolution limit [Å] | 2.150 | 9.620 | 2.150 |
| Rmerge | 0.095 | 0.044 | 1.714 |
| Rmeas | 0.097 | 0.045 | 1.746 |
| Rpim | 0.020 | 0.010 | 0.335 |
| Total number of observations | 2352015 | 22646 | 187391 |
| Number of reflections | 93392 | 1205 | 6891 |
| <I/σ(I)> | 21.5 | 47.7 | 2.5 |
| Completeness [%] | 98.9 | 97 | 100 |
| Redundancy | 25.2 | 18.8 | 27.2 |
| CC(1/2) | 0.999 | 0.999 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.12 M Monosaccharides (20 mM D-Glucose; 20 mM D-Mannose; 20 mM D-Galactose; 20 mM L-Fucose; 20 mM D-Xylose; 20 mM N-Acetyl-D-Glucosamine), 0.1 M buffer system 1 pH 6.5 (sodium HEPES and MOPS), and 50 % Precipitant Mix 2 (40% v/v Ethylene glycol; 20 % w/v PEG 8000) |
