8HUF

B28 in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	106.361, 106.361, 304.641
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.620 - 2.290
R-factor	0.19269
Rwork	0.191
R-free	0.22180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7cnd
RMSD bond length	0.008
RMSD bond angle	1.481
Data reduction software	HKL-3000
Data scaling software	Aimless (0.7.4)
Phasing software	MOLREP
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	152.350	152.350	2.350
High resolution limit [Å]	2.290	10.240	2.290
Rmerge	0.186	0.062	2.518
Rmeas	0.190	0.064	2.576
Rpim	0.037	0.014	0.532
Total number of observations	2097123	21405	136230
Number of reflections	79793	1085	5792
<I/σ(I)>	13.1	23.4	2.7
Completeness [%]	100.0	100	100
Redundancy	26.3	19.7	23.5
CC(1/2)	0.995	0.997	0.957

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.12 M Monosaccharides (20 mM D-Glucose; 20 mM D-Mannose; 20 mM D-Galactose; 20 mM L-Fucose; 20 mM D-Xylose; 20 mM N-Acetyl-D-Glucosamine), 0.1 M buffer system 1 pH 6.5 (sodium HEPES and MOPS), and 50 % Precipitant Mix 2 (40% v/v Ethylene glycol; 20 % w/v PEG 8000)