8HSV

The structure of rat beta-arrestin1 in complex with a rat Mdm2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0000
Spacegroup name	P 32
Unit cell lengths	80.673, 80.673, 173.555
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.340 - 3.000
R-factor	0.1786
Rwork	0.176
R-free	0.20850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.315
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.050
High resolution limit [Å]	3.000	8.130	3.000
Rmerge	0.107	0.047	0.844
Rmeas	0.113	0.049	0.887
Rpim	0.035	0.015	0.274
Total number of observations	266391
Number of reflections	25258	1270	1246
<I/σ(I)>	5.3
Completeness [%]	100.0	99.8	100
Redundancy	10.5	11.4	10.5
CC(1/2)	0.994	0.999	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	1M ammonium sulfate, 0.1M HEPES pH 9.5, 5mM n-dodecyl-b-iminodipropionic acid, monosodium salt