8HRZ

Crystal structure of the p97-N/D1 hexamer in complex with six p47-UBX domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-14
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	170.780, 178.850, 171.330
Unit cell angles	90.00, 119.80, 90.00

Refinement procedure

Resolution	40.013 - 2.700
Rwork	0.213
R-free	0.25580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.663
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0411)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.013	2.750
High resolution limit [Å]	2.700	2.700
Rpim	0.038	0.540
Number of reflections	242685	11034
<I/σ(I)>	9.4
Completeness [%]	99.3
Redundancy	7.4
CC(1/2)	0.999	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	14% PEG 3350, 0.2M ammonium citrate tribasic (pH 7), 0.01M hexamine cobalt (III) chloride