8HQ6

KL2 in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	104.752, 104.752, 302.806
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	99.190 - 2.030
R-factor	0.1927
Rwork	0.191
R-free	0.22414
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.544
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	104.720	2.080
High resolution limit [Å]	2.030	2.030
Rmerge	0.097	0.944
Rmeas	0.100	0.986
Rpim	0.020	0.275
Total number of observations	2544794	96031
Number of reflections	109668	7943
<I/σ(I)>	14.4	2.2
Completeness [%]	100.0
Redundancy	23.2	12.1
CC(1/2)	0.999	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.12 M Monosaccharides (20 mM D-Glucose; 20 mM D-Mannose; 20 mM D-Galactose; 20 mM L-Fucose; 20 mM D-Xylose; 20 mM N-Acetyl-D-Glucosamine), 0.1 M buffer system 1 pH 6.5 (sodium HEPES and MOPS), and 50 % Precipitant Mix 2 (40% v/v Ethylene glycol; 20 % w/v PEG 8000)