8HQ3

KL1 in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 43 21 2
Unit cell lengths	105.079, 105.079, 305.368
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 2.100
R-factor	0.17791
Rwork	0.176
R-free	0.21040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.211
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	105.100	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.139	1.396
Rmeas	0.142	1.442
Rpim	0.028	0.355
Total number of observations	2448394	119720
Number of reflections	100702	7326
<I/σ(I)>	10.7	2.2
Completeness [%]	100.0
Redundancy	24.3	16.3
CC(1/2)	0.968	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.12 M Monosaccharides (20 mM D-Glucose; 20 mM D-Mannose; 20 mM D-Galactose; 20 mM L-Fucose; 20 mM D-Xylose; 20 mM N-Acetyl-D-Glucosamine), 0.1 M buffer system 1 pH 6.5 (sodium HEPES and MOPS), and 50 % Precipitant Mix 2 (40% v/v Ethylene glycol; 20 % w/v PEG 8000)