8HQ1

Crystal Structure Of Human Lgi1-Adam22 Complex In Space Group C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97800
Spacegroup name	C 1 2 1
Unit cell lengths	373.503, 232.369, 80.035
Unit cell angles	90.00, 94.97, 90.00

Refinement procedure

Resolution	37.920 - 4.170
R-factor	0.268
Rwork	0.266
R-free	0.31400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w8a 5sxm 3g5c
RMSD bond length	0.004
RMSD bond angle	0.919
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.310
High resolution limit [Å]	4.170	4.170
Rmerge	0.074	0.758
Number of reflections	50134	4928
<I/σ(I)>	19.667	1.909
Completeness [%]	99.5	98.1
Redundancy	3.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	2M SODIUM CHLORIDE, 0.1M POTASSIUM/SODIUM PHOSPHATE pH=6.2