8HPC
Crystal structure of C171A Mutant of N-carbamyl-D-amino acid amidohydrolase complexed with N-carbamyl-D-Hydroxyphenylglycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-30 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 1 |
| Unit cell lengths | 79.165, 79.865, 91.070 |
| Unit cell angles | 91.40, 98.48, 92.78 |
Refinement procedure
| Resolution | 46.590 - 2.520 |
| R-factor | 0.21987 |
| Rwork | 0.218 |
| R-free | 0.25324 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.436 |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.590 | 2.570 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.140 | 0.433 |
| Number of reflections | 72971 | 3915 |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 98.0 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG3350, BIS-TRIS, Ammonium acetate, calcium chloride |
