8HOG
Crystal structure of Bcl-2 in complex with sonrotoclax
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.560, 47.549, 101.084 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.540 - 1.800 |
| R-factor | 0.2175 |
| Rwork | 0.216 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vau |
| Data reduction software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.540 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.059 | 0.533 |
| Number of reflections | 15163 | 1467 |
| <I/σ(I)> | 21.5 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.4 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M Bis-tris propane pH 9.0, 30% PEG 6000 |
