8HN4

Complex structure of HLA2402 with recognizing SARS-CoV-2 epitope QYIKWPWYI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97902
Spacegroup name	P 21 21 21
Unit cell lengths	49.066, 67.246, 116.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.640 - 2.853
Rwork	0.212
R-free	0.25060
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.640	2.950
High resolution limit [Å]	2.853	2.853
Rmerge	0.131	0.566
Rmeas	0.186	0.186
Rpim	0.083	0.345
Number of reflections	9438	901
<I/σ(I)>	9.53	2.06
Completeness [%]	99.4	98.79
Redundancy	4.8
CC(1/2)	0.978	0.801

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	287	30% PEG 4000, 0.2 M Sodium acetate, and 0.1 M Tris-HCl pH8.5