8HLT
The co-crystal structure of DYRK2 with YK-2-99B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 289.15 |
| Detector technology | PIXEL |
| Collection date | 2022-07-31 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.820, 60.670, 155.340 |
| Unit cell angles | 90.00, 113.08, 90.00 |
Refinement procedure
| Resolution | 47.850 - 2.800 |
| R-factor | 0.2131 |
| Rwork | 0.209 |
| R-free | 0.26690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.255 |
| Data reduction software | HKL-2000 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (19.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.850 | 2.810 |
| High resolution limit [Å] | 2.740 | 2.740 |
| Rmerge | 0.122 | 1.017 |
| Rmeas | 0.132 | 1.098 |
| Rpim | 0.051 | 0.410 |
| Total number of observations | 198388 | 14840 |
| Number of reflections | 29520 | 2126 |
| <I/σ(I)> | 9.9 | 2 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.7 | 7 |
| CC(1/2) | 0.996 | 0.871 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.05M HEPES pH7.0, 12%PEG 3350, 1% Trptone, 1mM NaN3 |
