8HL6

Crystal structure of human valosin-containing protein methyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 31 2 1
Unit cell lengths	58.170, 58.170, 129.291
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.748 - 1.800
R-factor	0.1935
Rwork	0.192
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.923
Data reduction software	MOSFLM
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.600	1.900
High resolution limit [Å]	1.800	1.800
Rpim	0.023	0.683
Number of reflections	23428	2011
<I/σ(I)>	17.2
Completeness [%]	93.2
Redundancy	9.4
CC(1/2)	0.999	0.405

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20 % PEG 1000, 0.1 M Na/K phosphate (pH 6.2), and 0.2M sodium chloride