8HGK
Crystal structure of human ClpP in complex with ZK53
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.310, 97.000, 123.840 |
| Unit cell angles | 90.00, 93.98, 90.00 |
Refinement procedure
| Resolution | 20.530 - 1.900 |
| R-factor | 0.1874 |
| Rwork | 0.186 |
| R-free | 0.22106 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tg6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.329 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (v5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.530 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.110 | |
| Number of reflections | 223016 | 223016 |
| <I/σ(I)> | 10.4 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2M Magnesium acetate tetrahydrate, 20% w/v Polyethylene glycol 3350, pH7.9 |
