8HCJ

Structure of GH43 family enzyme, Xylan 1, 4 Beta- xylosidase from pseudopedobacter saltans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	273
Detector technology	PIXEL
Collection date	2022-07-06
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.96546
Spacegroup name	P 1
Unit cell lengths	73.340, 89.662, 138.437
Unit cell angles	102.73, 93.98, 98.24

Refinement procedure

Resolution	134.290 - 2.566
Rwork	0.163
R-free	0.20910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nov
RMSD bond length	0.011
RMSD bond angle	1.494
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	134.290	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.080	0.020
Rmeas	0.100	0.280
Rpim	0.070	0.190
Number of reflections	103346	5300
<I/σ(I)>	0.04
Completeness [%]	95.0	97
Redundancy	1.92	2.01
CC(1/2)	0.940	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293.15	18% PEG 3350, 5% PROPANOL, 0.15M IMIDAZOLE