8HBS
Crystal of rAlfNmt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-08-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 |
| Unit cell lengths | 97.823, 97.823, 47.116 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.750 - 1.780 |
| R-factor | 0.2064 |
| Rwork | 0.205 |
| R-free | 0.23670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cav |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.065 |
| Data reduction software | PROCOR |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.750 | 1.847 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 42043 | 3384 |
| <I/σ(I)> | 22.73 | |
| Completeness [%] | 98.3 | |
| Redundancy | 2 | |
| CC(1/2) | 0.999 | 0.828 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M Tris, pH7.5, 20%(w/v) PEG 8000 |
