8H9D
Crystal structure of Cas12a protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-04-25 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 235.701, 85.628, 139.303 |
| Unit cell angles | 90.00, 110.15, 90.00 |
Refinement procedure
| Resolution | 29.980 - 3.100 |
| R-factor | 0.2329 |
| Rwork | 0.232 |
| R-free | 0.25240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5id6 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.795 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 51432 | 3567 |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 10 mM MgCl2, 0.1 M Sodium cacodylate pH 6.8, 17% PEG1000 |
