8H7A
Crystal structure of the dimer form KAT6A WH domain with its bound double stranded DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-09-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.007, 40.060, 81.252 |
| Unit cell angles | 90.12, 89.99, 113.88 |
Refinement procedure
| Resolution | 27.230 - 1.920 |
| R-factor | 0.1969 |
| Rwork | 0.195 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7y43 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.956 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.920 | 1.930 |
| Rmerge | 0.046 | 0.399 |
| Number of reflections | 33965 | 1640 |
| <I/σ(I)> | 20.97 | |
| Completeness [%] | 97.6 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.01 M Magnesium chloride hexahydrate, 0.005 M Nickel(II) chloride hexahydrate, 0.1 M HEPES sodium pH 7.0, 15% w/v Polyethylene glycol 3,350 |
