8H73
Crystal structure of antibody scFv against M2e Influenza peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 175.030, 175.030, 45.926 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.950 - 1.910 |
| R-factor | 0.1814 |
| Rwork | 0.180 |
| R-free | 0.20060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8h3b |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.087 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Number of reflections | 31735 | 2923 |
| <I/σ(I)> | 18.1 | 2.1 |
| Completeness [%] | 97.9 | 94.5 |
| Redundancy | 12.8 | |
| CC(1/2) | 0.999 | 0.694 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 4% MPD, 0.2M Citric acid pH 3.5, 20% PEG 1500 |
