8H68

Crystal structure of Caenorhabditis elegans NMAD-1 in complex with NOG and Mg(II)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	50.026, 91.867, 133.302
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.840 - 2.200
R-factor	0.1856
Rwork	0.182
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rzl
RMSD bond length	0.009
RMSD bond angle	0.942
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	75.640	75.640	2.060
High resolution limit [Å]	2.010	8.990	2.010
Rmerge	0.091	0.031	1.182
Rmeas	0.108	0.038	1.411
Rpim	0.059	0.021	0.759
Total number of observations		1445	10112
Number of reflections	31436	511	3017
<I/σ(I)>	8.8	28.9	0.9
Completeness [%]	98.9	95	99.8
Redundancy	3.2	2.8	3.4
CC(1/2)	0.995	0.996	0.544

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M HEPES, pH 7.5, 10% (w/v) PEG 6000, 10% (v/v) MPD