8H65

Crystal structure of human GCN5 histone acetyltransferase domain bound with butyryl-CoA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	77
Detector technology	CCD
Collection date	2015-10-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 21 3
Unit cell lengths	175.695, 175.695, 175.695
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.730 - 3.000
R-factor	0.1883
Rwork	0.185
R-free	0.25450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5trm
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.050
High resolution limit [Å]	3.000	8.130	3.000
Rmerge	0.138	0.048	0.515
Rmeas	0.160	0.055	0.596
Rpim	0.080	0.028	0.296
Total number of observations	132620
Number of reflections	35776	1850	1780
<I/σ(I)>	5.1
Completeness [%]	98.3	95.3	98.8
Redundancy	3.7	3.5	3.8
CC(1/2)		0.996	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.6M Ammonium sulfate, 0.1M Sodium acetate pH 4.6