8H5D
Crystal structure of YojK mutant in complex with UDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-02 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.753, 79.979, 99.244 |
| Unit cell angles | 90.00, 91.10, 90.00 |
Refinement procedure
| Resolution | 62.350 - 2.500 |
| R-factor | 0.1834 |
| Rwork | 0.179 |
| R-free | 0.27250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7vm0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.698 |
| Data reduction software | XDS |
| Data scaling software | SAINT (V8.38A) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 62.350 | 5.381 | 2.587 |
| High resolution limit [Å] | 2.498 | 4.271 | 2.498 |
| Number of reflections | 25482 | 5175 | 25482 |
| <I/σ(I)> | 12.8 | ||
| Completeness [%] | 99.8 | ||
| Redundancy | 9.51 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M Ammonium acetate, 0.1 M HEPES (pH 7.5) and 25% (w/v) Polyethylene glycol 3350 |
