8H4O

Crystal Structure of nucleotide-free Irgb6_T95D mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	71.532, 80.613, 147.649
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.550 - 2.050
R-factor	0.2277
Rwork	0.226
R-free	0.28280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vex 7ves
RMSD bond length	0.008
RMSD bond angle	0.886
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459: ???)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.170
High resolution limit [Å]	2.050	2.050
Number of reflections	54351	16439
<I/σ(I)>	10.25
Completeness [%]	99.4
Redundancy	6.95
CC(1/2)	0.998	0.508

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	Sodium malonate, PEG3350