8H4M

Crystal Structure of GTP-bound Irgb6_T95D mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	69.122, 75.750, 80.277
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.880 - 1.680
R-factor	0.2069
Rwork	0.206
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7vex
RMSD bond length	0.008
RMSD bond angle	0.921
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459: ???)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.780
High resolution limit [Å]	1.670	1.680
Number of reflections	49012	14962
<I/σ(I)>	11.55
Completeness [%]	99.8
Redundancy	6.9
CC(1/2)	0.998	0.475

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Sodium sulfate, polyethylene glycol 3350