8H0R
Crystal structure of a cataract-causing crystallin mutant (mouse CRYBB1 Y202X)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-07-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.919, 48.437, 113.321 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.103 - 1.201 |
R-factor | 0.1855 |
Rwork | 0.185 |
R-free | 0.19430 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.890 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.240 |
High resolution limit [Å] | 1.200 | 2.590 | 1.200 |
Rmerge | 0.058 | 0.050 | 0.482 |
Rmeas | 0.061 | 0.052 | 0.543 |
Rpim | 0.018 | 0.016 | 0.240 |
Total number of observations | 712380 | ||
Number of reflections | 68183 | 7241 | 5807 |
<I/σ(I)> | 17.5 | ||
Completeness [%] | 98.1 | 98.5 | 84.5 |
Redundancy | 10.4 | 10.5 | 4.6 |
CC(1/2) | 0.998 | 0.846 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 289 | PEG 3350 |