8GZF
Crystal Structure of METTL9-SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-25 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 61 |
| Unit cell lengths | 109.807, 109.807, 120.344 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.680 - 2.500 |
| R-factor | 0.1928 |
| Rwork | 0.190 |
| R-free | 0.24090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ezg |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.566 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.15.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.998 | 0.063 | 0.919 |
| Total number of observations | 482311 | ||
| Number of reflections | 55801 | 2768 | 2752 |
| <I/σ(I)> | 7.8 | ||
| Completeness [%] | 100.0 | 99.4 | 100 |
| Redundancy | 8.6 | 8.4 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 4% v/v glycerol, 1.8 M NaCl |
