8GYE
Crystal Structure of the 4-1BB in complex with ZG033 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.021, 65.913, 83.643 |
| Unit cell angles | 90.00, 82.50, 74.82 |
Refinement procedure
| Resolution | 19.910 - 2.300 |
| R-factor | 0.2248 |
| Rwork | 0.222 |
| R-free | 0.27660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4edw |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.533 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.910 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.085 | 0.821 |
| Number of reflections | 52988 | 5290 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 97.2 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 287 | 18% (w/v) PEG4000, 0.1M Tris pH8.0 |
