8GT4
Crystal structure of human cardiac alpha actin Q137A mutant (AMPPNP state) in complex with fragmin F1 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AichiSR BEAMLINE BL2S1 |
Synchrotron site | AichiSR |
Beamline | BL2S1 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2018-08-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.12 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.028, 92.817, 116.683 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.390 - 1.550 |
R-factor | 0.184 |
Rwork | 0.183 |
R-free | 0.20950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7w50 |
Data reduction software | XDS (Feb 5, 2021) |
Data scaling software | XSCALE (Feb 5, 2021) |
Phasing software | MOLREP (11.6.03) |
Refinement software | PHENIX (1.19.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.390 | 49.390 | 1.650 |
High resolution limit [Å] | 1.550 | 4.640 | 1.550 |
Rmerge | 0.090 | 0.046 | 1.077 |
Rmeas | 0.097 | 0.050 | 1.172 |
Total number of observations | 599841 | ||
Number of reflections | 89716 | 3625 | 14225 |
<I/σ(I)> | 12.54 | 37.57 | 1.63 |
Completeness [%] | 99.6 | 99.6 | 98.8 |
Redundancy | 6.686 | 6.756 | 6.217 |
CC(1/2) | 0.998 | 0.997 | 0.619 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG3350, Disodium Hydrogenphosphate, HEPES |