8GRF

Crystal structure of F-box protein in the ternary complex with adaptor protein Skp1(DL) and its substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-24
Detector	RAYONIX MX300HE
Wavelength(s)	0.900
Spacegroup name	P 21 21 21
Unit cell lengths	77.407, 150.976, 160.384
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.640 - 2.530
R-factor	0.2164
Rwork	0.215
R-free	0.24770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.426
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.640	2.620
High resolution limit [Å]	2.530	2.530
Number of reflections	63124	6158
<I/σ(I)>	24.58	4.09
Completeness [%]	99.2
Redundancy	6.9
CC(1/2)	0.997	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	70 mM Na citrate pH 5.5, 10.5% (w/v) PEG 6000