8GRE
F-box protein in complex with skp1(FL) and substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-26 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.900 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.589, 150.942, 160.286 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.710 - 2.300 |
R-factor | 0.2071 |
Rwork | 0.206 |
R-free | 0.23230 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.430 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.710 | 2.382 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 84031 | 8307 |
<I/σ(I)> | 21.9 | 3.28 |
Completeness [%] | 99.6 | |
Redundancy | 5.3 | |
CC(1/2) | 0.998 | 0.772 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 200 mM Na acetate, 100 mM Na citrate pH 5.5, 10% (w/v) PEG 4000 |