8GPK

Crystal structure of human anti-HIV-1 broadly neutralizing antibody F6 Fab

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	91.245, 62.271, 97.843
Unit cell angles	90.00, 105.18, 90.00

Refinement procedure

Resolution	19.710 - 3.340
R-factor	0.2455
Rwork	0.242
R-free	0.28060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bmf
RMSD bond length	0.003
RMSD bond angle	0.741
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.710	3.500
High resolution limit [Å]	3.300	3.300
Rmeas	0.240
Rpim	0.130
Number of reflections	29265	1352
<I/σ(I)>	6.11
Completeness [%]	97.4
Redundancy	6.6
CC(1/2)	0.970	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M sodium citrate, 20% PEG 3,350.