8GOA
Crystal Structure of Glycerol Dehydrogenase in the absence of NAD+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-12-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 161.470, 161.470, 291.907 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.240 - 2.900 |
R-factor | 0.1937 |
Rwork | 0.192 |
R-free | 0.21990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5zxl |
RMSD bond length | 0.008 |
RMSD bond angle | 0.914 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.240 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 86081 | 4204 |
<I/σ(I)> | 6.8 | |
Completeness [%] | 99.6 | |
Redundancy | 8.7 | |
CC(1/2) | 0.990 | 0.322 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM sodium citrate (pH 5.6), 500 mM ammonium sulfate, and 1 M lithium sulfate |