8GN6
Crystallization of Sialidase from Porphyromonas gingivalis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 131.160, 131.160, 181.173 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 56.860 - 2.100 |
R-factor | 0.17548 |
Rwork | 0.173 |
R-free | 0.21597 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6myv |
RMSD bond length | 0.007 |
RMSD bond angle | 1.403 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MLPHARE |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.860 | 2.238 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 101168 | 101168 |
<I/σ(I)> | 48.4 | |
Completeness [%] | 95.8 | |
Redundancy | 15.3 | |
CC(1/2) | 0.999 | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 287.15 | 1.4M Ammonium tartrate dibasic,0.1M Tris PH 7 |