8GJZ

Structure of a STING receptor from S. pistillata Sp-STING1 bound to 2'3'-cUA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-12-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 65 2 2
Unit cell lengths	113.429, 113.429, 259.272
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.120 - 2.920
R-factor	0.1977
Rwork	0.196
R-free	0.21670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.480
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.120	3.100
High resolution limit [Å]	2.920	2.920
Number of reflections	22222	3490
<I/σ(I)>	9.7
Completeness [%]	99.8
Redundancy	10.9
CC(1/2)	0.995	0.607

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	3.4 M NaCl, 100 mM HEPES, pH 7.2