8GIF
Crystal structure of a designed single-component Plasmodium falciparum AMA1-RON2L insertion fusion immunogen 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.360, 62.920, 60.010 |
Unit cell angles | 90.00, 96.25, 90.00 |
Refinement procedure
Resolution | 19.710 - 2.100 |
R-factor | 0.1719 |
Rwork | 0.170 |
R-free | 0.21570 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.482 |
Data reduction software | XDS (v Feb 5, 2021) |
Data scaling software | XDS (v Feb 5, 2021) |
Phasing software | PHENIX (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.710 | 2.176 |
High resolution limit [Å] | 2.100 | 2.101 |
Rmerge | 0.069 | 0.166 |
Rmeas | 0.082 | 0.202 |
Rpim | 0.044 | 0.113 |
Number of reflections | 17236 | 1600 |
<I/σ(I)> | 12.07 | 5.41 |
Completeness [%] | 98.4 | 92.38 |
Redundancy | 3.2 | 2.8 |
CC(1/2) | 0.994 | 0.953 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.2 M Magnesium chloride, 0.1 M Tris (pH 8.5), 20 % (w/v) PEG 8000 |