8GHE
The structure of h12-LOX in tetrameric form bound to endogenous inhibitor oleoyl-CoA
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.05 |
| Number of Particle | 2736609 |
| Software | cryoSPARC,PHENIX,Gctf,UCSF Chimera |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
