8GH4

Complex of Adam 10 disentegrin cysteine rich domains with human monoclonal antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-25
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 2 21
Unit cell lengths	78.578, 85.690, 129.934
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.690 - 3.800
R-factor	0.2889
Rwork	0.288
R-free	0.31150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.651
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.870
High resolution limit [Å]	3.800	3.800
Rpim	0.121	0.451
Number of reflections	7903	305
<I/σ(I)>	7.67
Completeness [%]	86.9	67.8
Redundancy	2.7	2.4
CC(1/2)	0.988	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.1 M bycine, 20% PEG 10K