8GDV
Structure of M66I mutant of disulfide stabilized HIV-1 CA hexamer in complex with CPSF6 peptide and IP6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2022-07-01 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.0722 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 133.172, 133.172, 205.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.880 - 3.300 |
R-factor | 0.3094 |
Rwork | 0.307 |
R-free | 0.34730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7snq |
RMSD bond length | 0.003 |
RMSD bond angle | 0.544 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.880 | 3.418 |
High resolution limit [Å] | 3.300 | 3.300 |
Rpim | 0.131 | |
Number of reflections | 27382 | 2733 |
<I/σ(I)> | 6.56 | 1.41 |
Completeness [%] | 95.4 | 97.18 |
Redundancy | 7.4 | |
CC(1/2) | 0.987 | 0.798 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | 0.1 M Tris hydrochloride, 11% PEG 8000, 5% Glycerol |