8GCK
Crystal structure of the human CHIP-TPR domain in complex with a 6mer acetylated tau peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.116 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 45.745, 65.717, 77.237 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.745 - 1.368 |
R-factor | 0.193268926362 |
Rwork | 0.192 |
R-free | 0.20994 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.842 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | BALBES |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.750 | 1.417 |
High resolution limit [Å] | 1.368 | 1.368 |
Number of reflections | 49457 | 4695 |
<I/σ(I)> | 7.61 | |
Completeness [%] | 99.2 | 94.96 |
Redundancy | 11.8 | |
CC(1/2) | 0.996 | 0.406 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M CaCl2, 0.1 M HEPES pH 7.4, 25% PEG 3350 |