8GBV
Crystal structure of PC39-17A, an anti-HIV broadly neutralizing antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.03320 |
Spacegroup name | P 1 |
Unit cell lengths | 43.519, 48.969, 113.878 |
Unit cell angles | 93.07, 98.26, 102.49 |
Refinement procedure
Resolution | 47.630 - 2.080 |
R-factor | 0.1946 |
Rwork | 0.193 |
R-free | 0.23030 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.653 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.080 | 2.080 |
Rpim | 0.130 | 0.880 |
Number of reflections | 53284 | 2603 |
<I/σ(I)> | 7.2 | 1.2 |
Completeness [%] | 98.1 | 96.9 |
Redundancy | 4.4 | 4.4 |
CC(1/2) | 0.760 | 0.380 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 0.2 M calcium acetate, 0.1 M imidazole (pH 8), 10% w/v PEG 8000 |