8GBQ

Structure of RNF125 in complex with UbcH5b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	P 31 2 1
Unit cell lengths	56.181, 56.181, 164.347
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.870 - 1.740
R-factor	0.1848
Rwork	0.184
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.650	48.650	1.770
High resolution limit [Å]	1.740	9.040	1.740
Rmerge	0.062	0.042	1.263
Rmeas	0.064	0.044	1.310
Rpim	0.017	0.012	0.342
Total number of observations		3214	20365
Number of reflections	59049	289	1537
<I/σ(I)>	21.7	50.6	2
Completeness [%]	99.4	98.8	89
Redundancy	14.7	11.1	13.2
CC(1/2)	0.999	0.998	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	289	0.1 M CHES 9.5 20% PEG 8000