8G4K
Complex of TbRII mini protein binder bound to the TbRII ECD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-02-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.983, 57.175, 78.798 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.280 - 1.240 |
Rwork | 0.181 |
R-free | 0.19580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m9z |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.800 | 1.270 |
High resolution limit [Å] | 1.240 | 1.240 |
Rmerge | 0.052 | 0.898 |
Rmeas | 0.055 | |
Rpim | 0.018 | 0.662 |
Number of reflections | 61539 | 2727 |
<I/σ(I)> | 18.9 | 0.6 |
Completeness [%] | 95.7 | 53.4 |
Redundancy | 8.9 | 2.3 |
CC(1/2) | 1.000 | 0.425 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 300 | 20% PEG5000 MME, 0.2-0.4 M ammonium sulfate, 0.1 M Tris, pH 7.4, 16-32% glycerol |