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8G2O

Fab structure - Anti-ApoE-7C11 antibody

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRF BEAMLINE BL17U1
Synchrotron siteSSRF
BeamlineBL17U1
Temperature [K]100
Detector technologyPIXEL
Collection date2022-01-14
DetectorDECTRIS EIGER X 16M
Wavelength(s)0.9792
Spacegroup nameP 21 21 2
Unit cell lengths92.700, 62.270, 82.140
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution26.260 - 1.700
R-factor0.1873
Rwork0.186
R-free0.20390
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)5i1c
RMSD bond length0.009
RMSD bond angle1.117
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwareMOLREP
Refinement softwarePHENIX (1.18.1_3865)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]26.2601.760
High resolution limit [Å]1.7001.700
Rmerge0.0530.726
Rpim0.015
Number of reflections530635220
<I/σ(I)>27.41
Completeness [%]99.9
Redundancy13.213.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP829321% PEG3350, pH 8.0, 0.2 M sodium malonate

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