8G2H

Crystal Structure of PRMT4 with Compound YD1113

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	84.215, 84.215, 130.426
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.660 - 1.490
R-factor	0.17147
Rwork	0.171
R-free	0.17745
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.470
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.520
High resolution limit [Å]	1.490	1.490
Rmerge	0.065	0.945
Number of reflections	87534	4297
<I/σ(I)>	12.6	2
Completeness [%]	100.0	100
Redundancy	8.2	8.1
CC(1/2)	0.998	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2 M Calcium chloride dihydrate, 0.05 M HEPES sodium pH 7.5, 28% v/v Polyethylene glycol 400, 0.002 M Spermine