8G2F
Crystal Structure of PRMT3 with Compound II710
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-16 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 70.733, 70.733, 175.262 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.140 - 2.060 |
R-factor | 0.20883 |
Rwork | 0.207 |
R-free | 0.25598 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.358 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.090 | 0.841 |
Number of reflections | 27517 | 1125 |
<I/σ(I)> | 8.9 | 1.7 |
Completeness [%] | 97.4 | 81.6 |
Redundancy | 10.4 | 8.1 |
CC(1/2) | 1.000 | 0.915 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% PEG3350, 0.3 M Sodium Formate |