8G2F

Crystal Structure of PRMT3 with Compound II710

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-16
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	70.733, 70.733, 175.262
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.140 - 2.060
R-factor	0.20883
Rwork	0.207
R-free	0.25598
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.358
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.100
High resolution limit [Å]	2.060	2.060
Rmerge	0.090	0.841
Number of reflections	27517	1125
<I/σ(I)>	8.9	1.7
Completeness [%]	97.4	81.6
Redundancy	10.4	8.1
CC(1/2)	1.000	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20% PEG3350, 0.3 M Sodium Formate