8G2D

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with tylosin, mRNA, deacylated A- and E-site tRNAphe, and deacylated P-site tRNAmet at 2.70A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-05
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	209.360, 449.770, 616.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	254.370 - 2.700
R-factor	0.2268
Rwork	0.224
R-free	0.27730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.838
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	254.370	254.370	2.770
High resolution limit [Å]	2.700	12.070	2.700
Rmerge	0.163	0.042	1.159
Rmeas	0.186	0.048	1.356
Total number of observations	6279665
Number of reflections	1527540	16763	109267
<I/σ(I)>	7.32	21.99	0.99
Completeness [%]	97.2	90.2	94.3
Redundancy	4.111	4.591	3.538
CC(1/2)	0.983	0.994	0.205

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME